Plasmid

Part:BBa_K5160007:Design

Designed by: Guangbin An   Group: iGEM24_SZU-China   (2024-09-25)


TRV1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 446
    Illegal XbaI site found at 631
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 446
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 446
    Illegal BglII site found at 1029
    Illegal BglII site found at 5070
    Illegal XhoI site found at 1488
    Illegal XhoI site found at 5689
    Illegal XhoI site found at 6685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 446
    Illegal XbaI site found at 631
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 446
    Illegal XbaI site found at 631
    Illegal SpeI site found at 5573
    Illegal PstI site found at 4610
    Illegal PstI site found at 5596
    Illegal AgeI site found at 4009
    Illegal AgeI site found at 5151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1212
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 1988
    Illegal BsaI.rc site found at 3752
    Illegal BsaI.rc site found at 5429
    Illegal SapI site found at 863


Design Notes

1

Source

1

References